Anticancer effect of the extracts from Polyalthia evecta against human hepatoma cell line (HepG2)
10.1016/S2221-1691(12)60058-6
- Author:
Sasipawan MACHANA
;
Natthida WEERAPREEYAKUL
;
Sahapat BARUSRUX
- Publication Type:Journal Article
- Keywords:
Polyalthia evecta;
Apoptosis induction;
Cytotoxicity;
Anticancer activity;
Hepatoma Accepted 23 October 2011
- From:Asian Pacific Journal of Tropical Biomedicine
2012;(5):368-374
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the anticancer activity of Polyalthia evecta (P. evecta) (Pierre) Finet& Gagnep against human hepatoma cell line (HepG2). Methods: The anticancer activity was based on (a) the cytotoxicity against human hepatoma cells (HepG2) assessed using a neutral red assay and (b) apoptosis induction determined by evaluation of nuclei morphological changes after DAPI staining. Preliminary phytochemical analysis of the crude extract was assessed by HPLC analysis. Results: The 50% ethanol-water crude leaf extract of P. evecta (EW-L) showed greater potential anticancer activity with high cytotoxicity [IC50 = (62.8 ± 7.3)μg/mL] and higher selectivity in HepG2 cells than normal Vero cells [selective index (SI) = 7.9]. The SI of EW-L was higher than the positive control, melphalan (SI = 1.6) and the apoptotic cells (46.4 ± 2.6) % induced by EW-L was higher than the melphalan (41.6 ± 2.1)% (P<0.05). The HPLC chromatogram of the EW-L revealed the presence of various kinds of polyphenolics and flavonoids in it. Conclusions:P. evecta is a potential plant with anticancer activity. The isolation of pure compounds and determination of the bioactivity of individual compounds will be further performed.
