Expression of SFRP5 mRNA in Rat Skeletal Muscle after Contusion
10.3969/j.issn.1004-5619.2015.05.001
- VernacularTitle:SFRP5 mRNA在大鼠肌肉挫伤后的表达
- Author:
Sanqiang LI
;
Yanjun LIU
;
Xiyan ZHU
;
Qiuxiang DU
;
Yafang WANG
;
Yingyuan WANG
;
Junhong SUN
- Publication Type:Journal Article
- Keywords:
forensic pathology;
w ounds and injuries;
polym erase chain reaction;
skeletal m uscle;
RNA,m essenger;
secreted frizzled-related protein 5;
rats
- From:
Journal of Forensic Medicine
2015;(5):337-340
- CountryChina
- Language:Chinese
-
Abstract:
Objective T o investigate the relationship betw een the expression of secreted frizzled-related protein 5 (SFR P5) m RNA and the tim e interval after skeletal m uscle injury in rats by real-tim e PC R . Methods A total of ninety SD rats w ere random ly divided into the contusion groups at different tim es including 4 h, 8 h, 12 h, 16 h, 20 h, 24 h, 28 h, 32 h, 36 h, 40 h, 44 h, 48 h after contusion, incision groups at different tim es including 4h and 8h after incision and the control group. T he sam ples w ere taken from the contused zone at different tim e points. T he total RNA w as isolated from the sam ples and re-versely transcribed to analyze the expression levels of SFRP5 m RNA . Results C om pared to the control group, the expression of SFRP5 m RNA in contusion groups w ere dow n-regulated w ithin 48 h after con-tusion and reached the low est level at 20 h, and the expression of SFRP5 m RNA gradually increased from 20 h to 48 h after contusion. T he expression of SFRP5 m RNA in the incised groups w ere signifi-cantly low er than that of the contusion groups at 4 h after injury. A t the tim e of 8 h, the expression levels betw een the contusion and incision groups show ed no statistically significant difference. Conclusion It is suggested that SFRP5 m RNA analysis m ay show regular expression and can be a m arker for estim ation of skeletal m uscle injury age.
