Expression of EIIIA+ Fibronectin in Incised Wound of Rat’s Skin

VernacularTitle:EIIIA+纤维连接蛋白在大鼠皮肤切创的表达
Author: Meng HE ; Hai ZHAO ; Yiwen SHEN ; Aimin XUE ; Long CHEN ; Yunju GU ; Ziqin ZHAO
Publication Type:Journal Article
Keywords: forensic pathology; w ounds and injuries; skin; fibronectin; rats
From: Journal of Forensic Medicine 2014;(6):409-412
CountryChina
Language:Chinese
Abstract: Objective To explore the relationship between the expression of EIIIA + fibronectin in incised wound of rat’s skin and injury time. Methods The wounding model was established by cutting the dor-salskin of 48 adult SD rats. The rats were sacrificed atthe pre-setinjury time as immediately, 0. 5h, 1h, 2 h, 3 h, 4 h, 6 h, and 8 h. The skin sam ples were taken at the m argin of wound. The expression of the EIIIA + fibronectin was detected by im m unohistochem istry and W estern blotting and the relationship be-tween its expression and injury time was observed. Results The expression of EIIIA + fibronectin was not observed im m ediately. The basal cell of skin began to showpositive expression 0. 5 h after injury. W ith the extension of injury time, positive staining became stronger. The value of relative optical density was gradually increased with prolonged injury time by the W estern blotting analysis. Conclusion The expres-sion of EIIIA + fibronectin could be used for estimation of injury time in the early stage of skin injury.