Linkage Disequilibrium and Mutation Rate Analysis of Sixteen X-STR Loci

VernacularTitle:16个X-STR基因座的连锁不平衡检验和突变率调查
Author: Li LI ; Junhong LIU ; Ruxin ZHU ; Yuan LIN
Publication Type:Journal Article
Keywords: forensic genetics; X chrom osom e; short tandem repeats; linkage disequilibrium; m utation rate
From: Journal of Forensic Medicine 2014;(6):437-440
CountryChina
Language:Chinese
Abstract: Objective To assess the patterns oflinkage disequilibrium (LD ) of16 STR loci on X chrom o-som e and investigate the genetic stability. Methods G enom ic DNA samples extracted from blood stains from 500 unrelated individuals and 885 lineage m em bers from E astern C hinese H an population were genotyped through m ultiplex am plification using ID typerX-16 kit by our independent research followed by capillary electrophoresis. LD was assessed by PowerM arker v3.25 software and m utation rate of every locus was analyzed. Results LD were not found at the 16 X-STR loci. A llele m utations were observed at 10 loci. A m ong them , m utation rates of DXS6809 and DXS7132 were both up to 0.004 8. Conclusion W hen the 16 X-ST R loci included in ID typerX-16 kit were used for parentage testing, product princi-ples can be applied to calculate the likelihood, but m utation should be taken into consideration in the case that the genotypes do not m eet the genetic law(especially at DXS6809 and DXS7132).