Apoptosis in Lungs and Liver after Crush Injury of Hindlimbs in Rat
10.3969/j.issn.1004-5619.2015.02.003
- VernacularTitle:大鼠双后肢挤压伤后肺、肝细胞的凋亡
- Author:
Jie ZHAO
;
Huarong WANG
;
Jianheng BU
;
Min ZUO
;
Guozhong ZHANG
- Publication Type:Journal Article
- Keywords:
forensic pathology;
wounds and injuries;
crush;
apoptosis;
Bax;
Bcl-2;
caspase-3;
rats
- From:Journal of Forensic Medicine
2015;(2):88-92
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the process of apoptosis in lungs and liver induced by crushing hindlimbs of rat, and study the mechanismof crush injury. Methods The rat experimental model of hindlimbs crush injury was established. The cell apoptosis in lungs and liver was detected by TUNEL assay, and the expression of Bax, Bcl-2 and caspase-3 apoptin was examined by immunohistochemistry. Results C ompared with the control group, the partial muscle injury of rat’s hindlimbs was more serious with more apoptosis observed in lungs and liver (P<0.05). The expression of Bax was up-regulated and Bcl-2 was down-regulated, whereas caspase-3 expression was activated (P<0.05). Conclusion The cell apoptosis has increased significantly in lungs and liver after crush injury of hindlimbs in rat. The corre-lation factor released during tissue injury may mediate apoptosis process.
