Application of Ion Torrent PGMTM System in Detection of Fetal DNA in Maternal Plasma

VernacularTitle:Ion Torrent PGMTM系统检测孕妇血浆中胎儿游离DNA
Author: Yanan LIU ; Xueying ZHAO ; Yuan PING ; Qingwen XU ; Jiangping HUANG ; Kainan ZOU ; Huaigu ZHOU
Publication Type:Journal Article
Keywords: forensic genetics; Ychrom osom e; pregnant women; fetus; short tandem repeat sequences; Ion Torrent PGMTM System
From: Journal of Forensic Medicine 2015;(6):432-435
CountryChina
Language:Chinese
Abstract: Objective To explore the feasibility of detecting of Y-STR of fetal DNA in m aternal plasm a using Ion Torrent PGMTM System . Methods A total of 16 fetal DNA sam ples from m aternal plasm as (8 cases from 38 w eeks gestational age and 8 ones from 12 w eeks) w ere prepared and a m ultiplex assay w ith 7 STR loci (DYS390,DYS391,DYS393,DYS438,DYS437,DYS456,DYS635) w as designed for m ul-tiplex-PC R am plification. U sing Ion Torrent PGMTM System , the results of Y-STR sequences and capillary electrophoresis w ere obtained and com pared. Results Y-STR specific alleles w ere detected in the m ater-nal plasm a of all the pregnant w om en having m ale babies of second and third trim ester, w hich w ere higher than that detected by capillary electrophoresis. C onsistent Y-STR genotypes w ere observed betw een fetal DNA from m aternal plasm a and genom ic DNA from the new born babies. Conclusion B ased on Ion Torrent PGMTM System , the prenatal Y-STR detection m ethod m ay provide a high-sensitive and high-throughput choice for prenatal STR detection in forensic testing.