Application of Ion Torrent PGMTM System in Detection of Fetal DNA in Maternal Plasma
10.3969/j.issn.1004-5619.2015.06.005
- VernacularTitle:Ion Torrent PGMTM系统检测孕妇血浆中胎儿游离DNA
- Author:
Yanan LIU
;
Xueying ZHAO
;
Yuan PING
;
Qingwen XU
;
Jiangping HUANG
;
Kainan ZOU
;
Huaigu ZHOU
- Publication Type:Journal Article
- Keywords:
forensic genetics;
Ychrom osom e;
pregnant women;
fetus;
short tandem repeat sequences;
Ion Torrent PGMTM System
- From:Journal of Forensic Medicine
2015;(6):432-435
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the feasibility of detecting of Y-STR of fetal DNA in m aternal plasm a using Ion Torrent PGMTM System . Methods A total of 16 fetal DNA sam ples from m aternal plasm as (8 cases from 38 w eeks gestational age and 8 ones from 12 w eeks) w ere prepared and a m ultiplex assay w ith 7 STR loci (DYS390,DYS391,DYS393,DYS438,DYS437,DYS456,DYS635) w as designed for m ul-tiplex-PC R am plification. U sing Ion Torrent PGMTM System , the results of Y-STR sequences and capillary electrophoresis w ere obtained and com pared. Results Y-STR specific alleles w ere detected in the m ater-nal plasm a of all the pregnant w om en having m ale babies of second and third trim ester, w hich w ere higher than that detected by capillary electrophoresis. C onsistent Y-STR genotypes w ere observed betw een fetal DNA from m aternal plasm a and genom ic DNA from the new born babies. Conclusion B ased on Ion Torrent PGMTM System , the prenatal Y-STR detection m ethod m ay provide a high-sensitive and high-throughput choice for prenatal STR detection in forensic testing.
