Studies on the Killing Effect of γ Ray Irradiated Peripheral Blood Mononuclear Cells Combined with ApoG2 on Human Prostate Cancer PC-3 Cells

VernacularTitle:γ射线辐照外周血单个核细胞联合ApoG2体外杀伤PC-3细胞的效应
Author: Yanli BAI ; Weifeng GONG ; Zhaolong WANG ; Jing LI ; Shan LI ; Zhixin LIU ; Xianqing ZHANG
Publication Type:Journal Article
Keywords: peripheral blood cell; gamma ray; low dose radiation; prostate cancer; ApoG2
From: Chinese Journal of Medical Physics 2010;27(1):1676-1680
CountryChina
Language:Chinese
Abstract: Objective:To observe the killing effect of irradiated peripheral blood mononuclear cells (PBMCs) at low dose combined with apogossypolone (ApoG2) on cultured human prostate cancer PC-3 cells.Methods:Human PBMCs were irradated by gamma ray at 1 gray,the irradiation dose rate was 17 Gy/min.The experiment were divided into PC-3 tumor cell control group,PC-3 cells with irradiated and non-irradiated PBMCs co-culture groups,ApoG2 treatment group,irradiated PBMCs and ApoG2 co-treatment group.Acridine orange/ethidium bromide (AO/EB) staining and MTT method were used to observe the killing effect of PBMCs and/or ApoG2.Results:The killing activity of irradiated PBMCs group and ApoG2 treatment group were obviously increased and were higlaer than that of non-irradiated group (P＜0.05).The killing activity of combined group were much higher than that of irradiated group and ApoG2 treatment group (P ＜0.01 ).Conclusion:Irradiated PBMCs at low dose combined with ApoG2 can enhances the anti-tumor effects markedly.