Serum IgE in Guinea Pigs Died of Anaphylactic Shock and Its Forensic Signifi-cance When the Guinea Pig Cannot be Timely Refrigerated
10.3969/j.issn.1004-5619.2015.05.002
- VernacularTitle:过敏性猝死豚鼠不及时冷藏对血清IgE的影响及法医学意义
- Author:
Jun SU
;
Jian YU
;
Yong LIU
- Publication Type:Journal Article
- Keywords:
forensic pathology;
anaphylaxis;
im m unoglobulin E;
cold-storage;
guinea pigs
- From:
Journal of Forensic Medicine
2015;(5):341-342
- CountryChina
- Language:Chinese
-
Abstract:
Objective T o investigate the serum IgE w ith various postm ortem intervals (PMI) in guinea pigs due to sudden death from anaphylactic shock and to explore the effect of refrigeration of corpse on serum IgE level and its application value in forensic m edicine. Methods T he anim al death m odels of anaphylactic shock w ere established. T he corpses w ere preserved at room tem perature (20℃) for 6 h and then refrigerated at 4℃. T he serum w as sam pled at 6, 12, 24, 36 and 48 hours after death. T he IgE level of serum w as detected w ith ELISA . The control group w as also established. Results The serum IgE level had significant difference betw een the experim ental group and the control group (P<0.05). T here w as no significant difference am ong the experim ental groups at 6, 12, 24, 36 and 48 hours post-m ortem (P>0.05). Conclusion If the corpses w ere placed in 4℃ conditions 6 hours after anaphylactic death, the serum IgE still show s a good m arker w ithin 48 h for forensic investigation.
