Research on functions and mechanisms of MicroRNA-203 on migration of human hypopharyngeal carcinoma cells
10.11659/jjssx.01E015026
- VernacularTitle:MicroRNA-203对人下咽癌细胞转移能力的影响及其机制研究
- Author:
Li GAO
;
Ka BIAN
- Publication Type:Journal Article
- Keywords:
MicroRNA-203;
MEKK1;
hypopharyngeal carcinoma;
migration
- From:
Journal of Regional Anatomy and Operative Surgery
2015;(3):241-244
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the roles and underlying molecular mechanism of MicroRNA-203 on the migration of human hypo-pharyngeal carcinoma cells. Methods The potential MicroRNA-203 target genes were searched by bioinformatic miRNA target prediction tools and KEGG database,and a large number of candidates was identified. The MEKK1 was selected for further investigation. This gene is known to play a role in tumor metastasis. The MicroRNA-203’s binding sites in MEKK1’s mRNA 3’UTR were analyzed by luciferase report-er assays. Nextly,the protein expression of MEKK1 in Fadu-Lv-MicroRNA-203 cells was determined by Western blot assay. The regulation of MEKK1’s mRNA expression by MicroRNA-203 was analyzed by qRT-PCR. Transwell cell migration assays were performed to confirm the im-pact of MicroRNA-203 on hypopharyngeal carcinoma metastasis. Results The expression level of endogenous MicroRNA-203 was negatively correlated with the mRNA and protein expression levels of MEKK1 in hypopharyngeal carcinoma cells. Transwell migration assay results showed that MicroRNA-203 overexpression inhibited hypopharyngeal carcinoma cell migration ability. Furtherly,MEKK1 can promote hypo-pharyngeal carcinoma cell migration ability. Conclusion MEKK1 is a direct target of MicroRNA-203. MicroRNA-203 plays a role in hypo-pharyngeal carcinoma cell migration ability through MEKK1.
