Regulatory mechanism of Glil gene on human periodontal ligament stem cells on proliferation and osteogenic differentiation
10.11659/jjssx.03E015025
- VernacularTitle:Glil基因对人牙周膜干细胞增殖及成骨分化调控机制的研究
- Author:
Jinying LUO
;
Jianxin ZHONG
;
Shaobing SHU
;
Jie ZHANG
;
Can ZHU
;
Qian ZHANG
;
Ling TANG
;
Jixiang ZHOU
- Publication Type:Journal Article
- Keywords:
human periodontal ligament stem cells;
Glil;
proliferation;
osteogenic differentiation
- From:
Journal of Regional Anatomy and Operative Surgery
2015;(3):245-248
- CountryChina
- Language:Chinese
-
Abstract:
Objective To up-regulate the expression of Glil gene in periodontal ligament stem cells ( PDLSCs) and to explore the effect of Glil gene on PDLSCs proliferation and osteogenesis differentiation by establishing Glil gene adenovirus vectors. Methods Subcloned Glil to viral backbone vector Adtrack-CMV and transfered the established vector to 293T cells, which was to acquire the virus particles. Trans-fected aim cells,namely PDLSCs,with these virus. Detected its effect on PDLSCs proliferation with CCK-8 assay, and detected the expression of Glil and the bone-related markers ALP and Runx2 through Western blot. Results An adenovirus vector, which were over expressed Glil gene, was successfully constructed and transfected to PDLSCs. Compared with the empty vector group and normal group, the over expressed one had a much slower proliferation rate in CCK-8 assay (P=0. 003). Western blot showed that ALP and Runx2 can be overexpressing os-teogenic differentiated after PDLSCs successfully transfected with the Glil gene. Conclusion Over expressing Glil gene would lead to a much slower proliferation rate in the PDLSCs and an increase of the bone-related markers. It is concluded that Glil can enhance the osteogenic dif-ferentiation capacity in PDLSCs.
