Progesterone on the expression of ADAM10,Ob-R and the secretion of SLR,LEP in human chorionic tropho-blast cells
10.11659/jjssx.03E015018
- VernacularTitle:孕激素对人绒毛滋养层细胞表达ADAM10、Ob-R及分泌SLR、LEP的影响
- Author:
Lei GAN
;
Zhen LI
;
Xiaohua WU
;
Demei YING
;
Liping CHEN
- Publication Type:Journal Article
- Keywords:
progesterone;
a disintegrin and metalloprotease domain 10;
long form leptin receptor;
soluble leptin receptor;
leptin
- From:
Journal of Regional Anatomy and Operative Surgery
2015;(3):249-252
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the different concentrations of progesterone on the expression of a disintegrin and metalloprotease 10 (ADAM10),long form leptin receptor (Ob-R) and the secretion of soluble leptin receptor (SLR),leptin (LEP) in primary pregnancy human chorionic trophoblast cells. Methods Cultured primary human trophoblast cells and added in different concentrations of progesterone (0,100,150 and 200 ng/mL) for 24 hours. The relative expression of ADAM10 and Ob-R in the cells and the content of SLR and LEP in the supernatant were detected. Results With increasing concentrations of progesterone,early human trophoblast cells ADAM10 content gradual-ly decreased,the difference between the two groups was significant (P<0. 05). With increasing concentrations of progesterone,human chori-onic trophoblast cells in early pregnancy Ob-R expression levels increased, the difference was no statistically significant between each two groups (P>0. 05). SLR content of the cell supernatants as the concentration of progesterone increased and decreased,there are significant differences between each two groups (P>0. 05). LEP cell supernatant in each group with the increase of the concentration of progesterone concentration increased gradually between the two groups were significantly different(P>0. 05). Pearson’s test showed that the expression of expression SLR and LEP exists a significant negative correlation (R= -0. 949,P<0. 01). Conclusion Progesterone may influence the ex-pression of ADAM10,SLR and LEP by the regulation of leptin to participate GDM occurs.
