Study of proliferation of chondrocytes co-cultured with platelet rich gel
10.11659/jjssx.03E015014
- VernacularTitle:富血小板凝胶促软骨细胞增殖的体外实验研究
- Author:
Jian KANG
;
Wen YUAN
;
Zhengqi CHANG
;
Haining SUN
;
Xiuchun YU
- Publication Type:Journal Article
- Keywords:
platelet rich gel;
chondrocytes;
tissue engineering cartilage;
growth factor
- From:
Journal of Regional Anatomy and Operative Surgery
2015;(4):387-390
- CountryChina
- Language:Chinese
-
Abstract:
Objective Preparing platelet rich gel through two-times centrifugal technique and co-culturing chondrocytes with PRG, then observing the proliferation and gene expression of chondrocytes, in order to provide a favorable way to prepare tissue engineering cartilage. Methods Centrifugating venous blood of rabbit through two-times centrifugal technique to obtain platelet rich plasma( PRP) ,then detecting the concentration of various growth factor in PRP. Admixing PRP with chondrocytes of rabbit and activating them with activator. After co-culti-vation,the proliferation of chondrocytes through MTT method and expression of ACAN,CollagenⅡand SOX-9 through realtime-PCR were ob-served,and compared with common cultured chondrocytes. Results The concentrations of PDGF-AB,TGF-β1,IGF-1 and VEGF in PRG were significantly higher than those in blood(P<0. 05). After co-cultivation, the proliferation rate of chondrocytes and the expression of ACAN,Collagen Ⅱ and SOX-9 were significantly higher than that of common cultured chondrocytes(P<0. 05). Conclusion Co-culturing chondrocytes with PRG is able to promote the proliferation and gene expression of chondrocytes. We considered that it is a excellent method to construct tissue engineering cartilage.
