Characteristics and significance of interferon-induced protein with tetratricopetide repeats 1 expressed in radia-tion injury and infection stress
10.11659/jjssx.01E016087
- VernacularTitle:IFIT1在放射损伤与感染应激中表达的特点和意义
- Author:
Xiaoshu GUO
;
Yuhui HAO
;
Yongping SU
;
Junping WANG
;
Song WANG
;
Yang XU
- Publication Type:Journal Article
- Keywords:
radiation injury;
lipopolysaccharide;
IFIT1;
glucocorticoid receptors;
toxemia
- From:
Journal of Regional Anatomy and Operative Surgery
2016;25(5):323-326
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore characteristics and significance of interferon-induced protein with tetratricopetide repeats 1 expressed in radiation injury and infection stress.Methods RNA was extracted from Raw264.7 cell,3T3 cell and 10T1 /2 cell after 5 hours stimulated with 5 μg/mL LPS.At the same time,to set up normal control group (untreated by LPS),and RNA of IFIT1 was detected by RT-PCR.Total-ly 20 C57 /BL6 mice were randomly divided into 4 groups,namely 0 h group,1 h group,4 h group and 12 h group.The mice were given 12 Gray60Co full-body exposure once,then liver IFIT1 was detected by western blot.Results Stimulated with LPS for 5 hours,IFIT1 was in-duced expression in Raw264.7 cell,3T3 cell and 10T1 /2 cell.The expression of normal control group was negative.The level of IFIT1 /Actin increased significantly 1 hour after radiation injury,and it reached the peak 12 hours after radiation injury (P <0.01).Conclusion LPS can stimulated a variety of cell lines expressed IFIT1,prompting that IFIT1 may participate in the occurrence and development of post-traumatic toxemia.IFIT1 of liver tissue increased significantly during the early stage in radiation mice.
