Effect of amelogenin on the migration,adhesion and proliferation of periodontal ligament stem cells
10.11659/jjssx.04E016044
- VernacularTitle:釉原蛋白对人牙周膜干细胞迁移、粘附及增殖能力的影响
- Author:
Xi ZHAO
;
Yujia HAN
;
Yu XIONG
- Publication Type:Journal Article
- Keywords:
PDLSCs;
amelogenin;
migration;
adhesion;
proliferation
- From:
Journal of Regional Anatomy and Operative Surgery
2016;25(5):327-330
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of amelogenin (AML)on the migration,adhesion and proliferation of periodontal ligament stem cells (PDLSCs).Methods STRO-1 +PDLSCs were selected by Flow cytometer.The transwell system was used to detect the effect of AML on migration of PDLSCs.Cells attachment assay was used to detect the effect of AML on adhesion of PDLSCs.MTT was used to detect the effect of AML on the proliferation of PDLSCs.Results The results of transwell showed that AML could induce the migration of PDLSCs with positive correlation.Cells attachment assay showed that the adhesion of PDLSCs was effected by AML as well,and the effect was in-creased with the extension of incubation time.Results of MTT showed that AML could promote the proliferation of PDLSCs in a dose depend-ent manner.Conclusion AML could increase the proliferation,adhesion and migration potential of PDLSCs.
