Research on blood-brain barrier of estrogen in rats after cerebral ischemia-reperfusion
10.11659/jjssx.1672-5042.201403002
- VernacularTitle:雌激素对大鼠脑缺血再灌注后血脑屏障作用研究
- Author:
Jinyi HOU
;
Xia SHEN
;
Juguang SUN
- Publication Type:Journal Article
- Keywords:
estrogen;
BBB;
tight junction;
occludin;
focal cerebral ischemia-reperfusion model
- From:Journal of Regional Anatomy and Operative Surgery
2014;(3):224-228
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of estrogen on BBB permeability,occludin expression after ischemia-reperfusion in rats,and to make a further investigation on the role of estrogen in cerebral ischemia. Methods Ovariectomized rats were randomly divided into sham-operated group,model group,and estrogen pretreatment group. 4 h,24 h,3 d after ischemia-reperfusion were selected as different observation points,and changes of edema,occluding expression,and blood-brain barrier permeability of the 3 points in time were observed. BBB ultra-structure electron microscope observation was made at 24 h and 3 d after ischemia-reperfusion. Changes in cerebral edema were measured by brain water content percentage;protein expressions were measured by Western blot;and BBB permeability was measured by Evans blue as-say. Results Compared with the sham group,brain tissue water content and EB content in model group both increased 4 h after ischemia-reperfusion (P<0. 05),and they keep increasing as time goes by and finally reaching the peak (P<0. 01). Compared with the model group at the same time point,brain tissue water content and EB content in estrogen pretreatment group decreased in different degrees (P<0. 05 or P<0. 01),and it was most significant at the time point of 24 h after ischemia-reperfusion (P<0. 01). Compared with model group,through electron microscope observation,ia was displayed that BBB TJ's open was reduced in estrogen pretreatment group,astrocyte foot process and capillary tube edema were lighter,and it was significant at the time point of 3 d after ischemia-reperfusion. Western blot detection of occludin protein expression found that it was lower in the model group compared with the sham group at 4 h after ischemia-reperfusion,but there was no significant difference (P>0. 05). 24 h after ischemia-reperfusion,the occludin expression were weaker than that in the sham surgery group with a significant difference (P<0. 05),and it keep weakening with a significant difference 3 d after ischemia-reperfusion (P<0. 01). In estrogen group,there was no significant difference compared with the model group in terms of occludin expression at 4 h after ischemia-reper-fusion (P>0. 05). In estrogen group,there was significant difference in occludin protein expression at 24 h and 3 d after ischemia-reperfusion compared with model group at the same time point (P<0. 05). Conclusion BBB ultrastructure disclose TJ was broke and vesicles within EC was increased and astrocyte cell foot process was swelling in MCAO model,it might be the vasogenic brain edema pathological basis for MCAO. In MCAO animal model,with ischemia-reperfusion time increasing,TJ protein occludin expression significantly decreased,it suggests that occludin plays an important role in the regulation of TJ permeability. Estrogen increases has a very close relationship with occludin ex-pression,and it may be one of the mechanisms of protecting BBB integrity and lessening cerebral edema.
