Regulatory effect of bFGF and TGF-βfor the proliferation of mesenchymal progenitor cells derived from prima-ry osteoarthritis cartilage
10.11659/jjssx.1672-5042.201403009
- VernacularTitle:bFGF和TGF-β对原发性骨关节炎软骨间充质祖细胞增殖调控作用
- Author:
Jun LIU
;
Hongwei WANG
;
Yu CHEN
;
Hailong YU
;
Qi WANG
;
Huifeng YANG
;
Junxiong MA
;
Liangbi XIANG
- Publication Type:Journal Article
- Keywords:
osteoarthritis;
mesenchymal progenitor cells;
bFGF;
TGF-β
- From:
Journal of Regional Anatomy and Operative Surgery
2014;(3):244-246
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the regulatory effect of bFGF and TGF-β1 for the proliferation of mesenchymal progenitor cells ( MPCs) derived from primary osteoarthritis cartilage, and to provide theoretical evidence in preventing and curing primary OA. Methods Different concentrations of bFGF and TGF-β1 ( alone or combined) were used to treat primary OA cartilage and their effects on proliferation of MPCs were tested by MTT method. Results Either bFGF (10. 0~50. 0 ng/mL) or TGF-β1 (0. 1 ~1. 0 ng/mL) alone can significantly promote the proliferation of MPCs derived from primary OA cartilage (P<0. 05). But with their increased concentration,the proliferation rate was of no significant changes (P>0. 05). The combination of 10. 0 ng/mL bFGF and 1. 0 ng/mL TGF-β1 significantly increased the prolifer-ation of MPCs from primary OA (P<0. 05). Conclusion Both bFGF and TGF-β11 play important roles in the proliferation of MPCs in primary OA cartilage,and they can increase the proliferation in different degree with different concentrations. There must be feasible methods of gene technology to promote cell proliferation and differentiation of MPCs for repairing articular car-tilage injury.
