Effect of human tumor necrosis factor alpha-sercreting on proliferation of LOVO colon cancer cells

VernacularTitle:构建hTNF-α/293基因细胞对人结肠癌细胞增殖影响的实验研究
Author: Yuhong GAO ; Yilong XUE ; Jingkun PAN
Publication Type:Journal Article
Keywords: LOVO cells; human tumor necrosis factor-α; human embryonic kidney cell
From:Journal of Regional Anatomy and Operative Surgery 2013;(6):591-593
CountryChina
Language:Chinese
Abstract: objective To observe the effects of co-culture of hTNFα-sercreting and human colon cancer cells LOVO on the proliferation of cancer cells. Methods The stable transfected hTNF-α/293 , mRNA of Hek-293 cell and protein expression were detected by RT-PCR and ELISA. The positive group was added hTNF-αfactor,and MTT assay was applied under the optical density 490 nm. Through human tumor cell proliferation inhibition experiment,the inhibitory effects on colon cancer cells ( LOVO) proliferation were observed. Results hTNF-α/293 cells and hTNF-α-positive group showed a significant lower A,which suggested that hTNF-α/293 cells and hTNF-α-positive group had significant inhibition on the proliferation of colon cancer cells. Conclusion The inhibition of hTNF-αsecreted by hTNF-α/293 cells on co-lon cancer cell proliferation shows significant dose-effect dependency,and hTNF-αexpresses a considerable inhibition on the colon cancer cell proliferation as positive drug.