Effect of low concentration of cerium oxide nanomaterials on hepatocellular carcinoma cell proliferation
10.11659/jjssx.01E016075
- VernacularTitle:低浓度氧化铈纳米材料对肝癌细胞增殖能力的影响
- Author:
Heng CHENG
;
Zhongli LIAO
;
Hongyan CHEN
;
Tao WANG
;
Hong GUO
- Publication Type:Journal Article
- Keywords:
cerium oxide;
hepatocellular carcinoma cell;
proliferation;
flow cytometry analysis
- From:Journal of Regional Anatomy and Operative Surgery
2016;25(4):244-247
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the impact of different low concentrations of cerium oxide for hepatocellular carcinoma cell prolifera-tion.Methods Three different types of hepatoma cells (Huh7, HepG2,7721) were cultured,and added different concentrations of cerium oxide (0.005,0.01,0.05,0.1,1 μg/mL),of which the cell proliferation was detected by CCK8.The apoptosis-related genes was detected by qRT-PCR technology.The cell cycle was analyzed by flow cytometry.And the effect of low concentration cerium oxide on hepatocellular carci-noma cells tumorigenicity was confirmed by the nude mice experiments.Results CCK8 experiment showed that low concentrations of cerium oxide could promote proliferation of hepatocellular carcinoma cell, especially in concentration of 0.01μg/mL.The qRT-PCR showed that low concentration of cerium oxide could inhibit the apoptosis of hepatocellular carcinoma cell.The flow cytometry analysis had not found any effect of cerium oxide on cell cycle.The tumorigenicity experiments confirmed that low concentrations of cerium oxide could enhance the tumorigenic ability of hepatocellular carcinoma cell.Conclusion Low concentration of cerium oxide can significantly improve the proliferation of liver cancer cells.
