Comparison of genomic DNA content of liver and spleen tissue for different age of elderly
10.11659/jjssx.11E014002
- VernacularTitle:老年、高龄及长寿老人肝脾组织基因组DNA含量的变化及比较
- Author:
Yazhuo HU
;
Zheng XIA
;
Zhitao HAN
;
Honghong ZHANG
;
Jianhua WANG
;
Miao GENG
;
Xiumei MENG
- Publication Type:Journal Article
- Keywords:
elderly;
liver;
spleen;
genomic DNA;
autopsy
- From:
Journal of Regional Anatomy and Operative Surgery
2015;(2):175-178
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the change of genomic DNA of liver and spleen tissue for different age of the elderly,and provide the experimental data for aging-related research. Methods 35 livers and 33 spleens of autopsied samples preserved in refrigerator at-80 ℃ were divided into 3 groups according to age:age 65y to 79y,age 80y to 89y,age≥90y. The content of DNA in liver and spleen was determined by ultraviolet absorbent method. Results Compaired with age 80y to 89y (0. 310 ± 0. 286)mg/mL,the content of DNA in liver was significant higher at age 65y to 79y (1.464 ±0.488)mg/mL and age ≥90y(1.147 ±0.333)mg/mL(P<0.05);Compared with age 80y to 89y(0. 938 ± 0. 589)mg/mL,the content of DNA in spleen was significant higher at age 65y to 79y(1. 723 ± 0. 726)mg/mL and age≥90y(1. 688 ± 0. 963)mg/mL(P<0. 05). The content of DNA was significant lower in liver (0. 856 ± 0. 658)mg/mL than that in spleen (1. 414 ± 0. 852)mg/mL. Conclusion The content of DNA in human liver and spleen tissue may be decrease along with aging. The content of DNA in the group at age≥90y may be increase. There were some differences between different viscera tissue in content of DNA.
