Role of AMPK signaling pathway in regulating antibacterial autophagy in murine bone marrow derived macro-phages
10.11659/jjssx.09E015136
- VernacularTitle:AMPK信号通路在调节小鼠骨髓巨噬细胞抗菌性自噬效应中的作用
- Author:
Shijun FAN
;
Yuanfeng ZHU
;
Yongjun YANG
;
Xin LIU
- Publication Type:Journal Article
- Keywords:
AMPK;
murine bone marrow derived macrophages ( BMDM);
antibacterial autophagy
- From:Journal of Regional Anatomy and Operative Surgery
2015;24(6):591-594
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulatory effects and possible mechanisms of autophagy induction and bactericidal activity in muring bone marrow derived macrophages ( BMDMs) . Methods Murine BMDMs were isolated and cultured in vitro. Then standard strain of E. coli was used to infect BMDMs and cellular autophagy levels and AMPK activation were detected. We next modulated functions of AMPK by pretreating cells with the specific agonist or antagonist of AMPK. Then cellular AMPK activation, autophagy levels and bactericidal activity were observed after E. coli infection. Results In E. coli infected BMDMs,autophagy related molecules like LC3B and Beclin1 were upregu-lated,accompanied with elevated LC3Ⅱ/Ⅰratio. Phosphorylation of AMPK was also upregulated by E. coli treatment. Enhanced AMPK activi-ty by its agonist leads to increased cellular autophagy and bactericidal activity,whereas inhibition of AMPK by its suppressor downregulated autophagy and dampened bactericidal activity. Conclusion AMPK and its related signaling pathway is required for anti-bacterial response in macrophage,which is dependent on its function in upregulating autophagy related molecules and inducing autophagy.
