Protective role of ghrelin in depleted uranium-induced damage of MC3 T3-E1 cells

VernacularTitle:饥饿素对贫铀所致MC3T3-E1细胞损伤的保护作用研究
Author: Yuhui HAO ; Jiawei HUANG ; Cong LIU ; Rong LI
Publication Type:Journal Article
Keywords: ghrelin; depleted uranium; osteoblast; reactive oxygen species
From: Journal of Regional Anatomy and Operative Surgery 2015;24(6):595-598
CountryChina
Language:Chinese
Abstract: Objective To evaluate the impact of ghrelin on depleted uranium ( DU)-induced damage of the osteoblast MC3T3-E1. Methods MC3T3-E1 cells were treated with different doses of ghrelin for 1 h before DU (500 μM) treatment. After 24 hours,the cell via-bility,intracellular tartrate-resistant acid phosphatase (StrACP),alkaline phosphatase (AKP),osteoprotegerin (OPG),solvable receptor acti-vator of nuclear factor-κB ligand ( sRANKL) ,catalase ( CAT) and reactive oxygen species ( ROS) were measured. Results After DU expo-sure,ghrelin pretreatment increased the cell viability and CAT levels,and reduced intracellular StrACP,AKP,sRANKL/OPG and ROS in a dose-dependent manner. Conclusion Through maintaining the balance of OPG/RANKL and reducing the oxidative stress,ghrelin could pro-tect against DU-induced damage of MC3T3-E1 cells.