The expression of V-ATPase and its significance in colon cancer
10.11659/jjssx.1672-5042.201405014
- VernacularTitle:V-ATPase在结肠癌中的表达及其临床意义
- Author:
Senlin ZHAO
;
Bin MENG
;
Dongwang YAN
;
Likang ZHANG
;
Feifei CUI
;
Huijun LU
;
Jian CHEN
;
Zhihai PENG
- Publication Type:Journal Article
- Keywords:
colon cancer;
vacuole type;
V-ATPase
- From:
Journal of Regional Anatomy and Operative Surgery
2014;(5):486-488
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of V-ATPase in colon cancer and its clinical significance. Methods Detecting the expression of V-ATPase mRNA in 20 paired of colon tumor tissues and normal tissues by using reverse transcription-polymerase chain reaction ( RT-PCR) and real-time quantitative polymerase chain reaction( Real-time PCR) , and testing the expression of V-ATPase protein by immu-nohistochemistry of EnVinsion. Results The expression of V-ATPase mRNA in tumor tissues and its paired normal tissues were (5. 37 ± 0. 44) and (2. 03 ± 0. 35)(P<0. 01). The positive immunohistochemistry of V-ATPase in tumor tissues and its paired normal tissues were 69. 1%(47/68) and 5. 8%(4/68) respectively, and the positive expression were primarily in cytoplasm and cytomembrane. Overexpression of V-ATPase was associated with tumor stage (P<0. 05), lymph node metastasis (P=0. 044), distant metastasis (P=0. 049), vessel in-vasion (P=0. 044) and differentiation (P<0. 001). Conclusion Overexpression of V-ATPase plays a significant role in the carcinogene-sis and the progression of colon cancer, which might be an important postoperative therapeutic target.
