Role of hyperpolarization-activated cyclic nucleotide-gated cation nonselective channel in spontaneous contrac-tion of smooth muscles in human isolated ureters
10.11659/jjssx.01E015035
- VernacularTitle:HCN通道在人体输尿管自发性收缩中的作用
- Author:
Guangui FENG
;
Qian LIU
;
Bishao SUN
;
Jingzhen ZHU
;
Zhou LONG
;
Longkun LI
- Publication Type:Journal Article
- Keywords:
HCN channel;
ureters;
spontaneous contraction
- From:Journal of Regional Anatomy and Operative Surgery
2015;(2):119-122
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effects of hyperpolarization-activated cyclic nucleotide-gated cation nonselective channel in the hu-man ureter on the spontaneous contraction of smooth muscles. Methods Four HCN subtypes were detected in human ureteral tissue using reverse transcription polymerase chain reaction,Western blotting and immunohistochemical. ZD7288,the HCN blocker, was used to observe the changes of ureteral muscle contraction amplitude and frequency by applying the ureteral smooth muscle strip test in vitro. Results HCN1-4 isoforms were all identified in human ureter using reverse transcription-polymerase chain reaction and Western blotting. Through the immunohistochemical,HCN channel was found mostly in the urothelium layer and muscular layer of human ureteral wall. ZD7288 significantly decreased the bladder excitation. Conclusion All 4 HCN channel hypotypes exist in the human ureter, and affect the ureteral excitation.
