Hypoxia enhances stemness of cancer stem cells in glioblastoma
10.11659/jjssx.01E015027
- VernacularTitle:缺氧增强胶质瘤干细胞“干性”的初步研究
- Author:
Pengcheng LI
;
Chun ZHOU
;
Lunshan XU
;
Hualiang XIAO
- Publication Type:Journal Article
- Keywords:
cancer stem cells;
stemness;
hypoxia;
glioblastoma
- From:
Journal of Regional Anatomy and Operative Surgery
2015;(5):479-483
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between hypoxia and stemness of cancer stem cells ( CSCs ) . Methods U87 cells,U251 cells and primary glioma cells were experienced hypoxia. Detected the ultrastructure of these cancer cells with transmission elec-tron microscopy;detect the cell growth with MTT assay;cell cycle and CD133 expression were detected by flow cytometry;and the cell mi-gration ablity were detected through transwell chamber assay;the colony-forming efficiency were deteced by colony-forming assay; and real-time quantitative PCR and Western blot were carried out to detect the mRNA and protein expression of markers of stem cells and their differ-entiation respectively. Results Hypoxia maintained the undifferentiated state of primary glioma cells, slowed down the growth of glioma cells which were in a relatively quiescent stage, increased the colony forming efficiency and migration of glioma cells, and increased the expression of markers of stem cells, but the expression of markers for stem cell differentiation was reduced after hypoxia treatment. Conclusion Hypoxi-a may induce the “dedifferentiation” of differentiated glioma cells which then acquire the stemness.
