Preliminary study on preparation of universal blood type through masking RBC’ s surface antigen with Tween
10.11659/jjssx.06E014388
- VernacularTitle:Tween遮蔽RBC表面抗原制备通用血型的初步研究
- Author:
Hongyu CHEN
;
Wei HAN
;
Heng JIANG
;
Xiaolin YIN
;
Hui LI
;
Yuqi LI
;
Chengshu WU
- Publication Type:Journal Article
- Keywords:
surface antigens on red blood cells;
Tween-20;
Tween-80;
chemical modification;
universal blood type
- From:
Journal of Regional Anatomy and Operative Surgery
2015;(1):60-62
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the shielding effect that Tween has made on the erythrocyte’ s surface antigen( ABO and D antigen mainly) and the stability of the RBC. Methods Various types red blood cells’ surface antigens were incubated with different concentrations of Tween,then the titers of RBC’ s surface antigen before and after incubation were compared. The erythrocyte’ s function alterations and sta-bility through the morphological obersavation,the osmotic fragility,RBC’ s own hemolysis rate,oxygen saturation( SO2 ) ,routine test of blood as well as the supernatant of free hemoglobin determination were confirmed. Results The masking effect of the Tween-20 on D antigen with the weaken titer keeping above“ +” was better and more stable than that on A,B antigen. The impact on B antigen was a little worse,and A was the worst. The advantaged concentration is about 0. 004%. Besides,the influence of Tween-80’s on B and D antigen was not apparent e-nough. Among all of the concentration,0. 74% and 0. 80% did a relatively better job,which also could keep the weaken titer above “ +”. Conclulsion The shielding effect that Tween has made on the erythrocyte’ s surface antigen( ABO and D antigen mainly) is stable,which keeps above “ +”. The morphology and function of the RBC does not change.