Effect of tumor necrosis factor-alpha preconditioning and ischemic preconditioning on hepatic ischemia-reperfu-sion injury in rats
10.11659/jjssx.09E014026
- VernacularTitle:肿瘤坏死因子-α预处理与缺血预处理在减轻肝脏缺血再灌注损伤的作用
- Author:
Huaibin GUO
;
Yan ZHAO
;
Feng LIU
;
Lihui YUE
;
Wanxing ZHANG
- Publication Type:Journal Article
- Keywords:
liver;
ischemia-reperfusion injury;
TNF-α;
ischemic preconditioning
- From:
Journal of Regional Anatomy and Operative Surgery
2015;(1):70-72
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the effect of TNF-α preconditioning and ischemic preconditioning on hepatic ischemia-reperfusion injury ( IRI) and investigate the underlying mechanisms of TNF-αpreconditioning. Methods Fourty healthy male Wistar rats were random-ly divided into four groups which were Sham-operated group ( SO) ,ischemia-reperfusion group ( IR group:produced by total inflow occlusion for 30 min) ,ischemic preconditioning group ( IPC group:induce with 10 min hepatic ischemic and open 10 min before IR) and TNF-αpre-conditioning group ( TPC group:intraperitoneal injection with 1 μg/kg TNF-a 30 min prior to IR) . The sample of blood and hepatic tissue of all groups were taken after experiment. The protein levels of NF-κBp65 and Bcl-2 in the hepatic tissue were detected by immunohistochemis-try. Results There was significant difference (P<0. 05) between IR group and IPC group,TPC group on the level of ALT,AST and the expression of NF-κBp65 and Bcl-2,apoptosis index in hepatic tissue. There was no significance difference (P>0. 05) between IPC group and TPC group. Conclusion TNF-α preconditioning decreased the intensity of hepatic IRI,just as ischemic preconditioning,by induces an de-crease in the NF-κBp65 expression and an increase in the Bcl-2 expression.
