Establishment of a rapid method for the isolation of murine peritoneal macrophages
10.11659/jjssx.11E014005
- VernacularTitle:小鼠腹腔巨噬细胞的快速提取及培养
- Author:
Shijun FAN
;
Xin LIU
;
Min HUANG
;
Ning WANG
;
Jiang ZHENG
- Publication Type:Journal Article
- Keywords:
BALB/c mice;
macrophage;
extraction;
culture;
identification
- From:
Journal of Regional Anatomy and Operative Surgery
2015;(2):130-131
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rapid method for the isolation of murine peritoneal macrophages. Methods Abdominal lavage was performed with NS and the peritoneal macrophages was purified from the lavage,which was later transferred into high glucose DMEM cul-ture medium. Cell viability was measured via the typan blue staining. Purity was observed via Wright's stain. Results High purity macropha-ges with typical morphology were obtained. Conclusion A simple and realistic method was set up for the isolation of murine peritoneal mac-rophages.
