Expression and significance of MMP-2,MMP-3 and MMP-9 in the gastric carcinoma
10.11659/jjssx.03E016107
- VernacularTitle:MMP-2、MMP-3和 MMP-9在胃癌患者中的表达及意义
- Author:
Yunpeng YANG
;
Li LIU
;
Hui LI
- Publication Type:Journal Article
- Keywords:
gastric carcinoma;
cancer cell metastasis;
MMP-2;
MMP-3;
MMP-9
- From:
Journal of Regional Anatomy and Operative Surgery
2016;25(9):625-628
- CountryChina
- Language:Chinese
-
Abstract:
Objecitive The research was in order to explore the roles of MMP-2,MMP-3 and MMP 9 which played in the gastric cancer cell metastasis,and make a further study of the clinical signifance.Methods Both SGC-7901 and GES-1 were cultured in vivo.The migra-tion were examined by Wound-healing and the Transwell assay.The expression of MMP-2,MMP-3 and MMP 9 in the medium were detected by ELISA.The positive rate of MMP-3 in the gastric cancer,gastritis and normal was used the IHC,and the serum of MMP-2,MMP-3 and MMP-9 were also examined by ELISA.Results Both Wound-healing and Transwell assay showed that the migration of SGC-7901 cells was more quicker than the GES-1.Comparing to GES-1,the MMP-2,MMP-3 and MMP-9 were higher in the SGC-7901.The immunohistochemical results showed that the positive rate of MMP-3 in the gastric cancer patients was 84.4%,it was higher than the latter.The serum levels of MMP-2 MMP-3 and MMP-9 were also significantly higher than others.Conclusion The migration of gastric cancer cells was depended on the MMP-2,MMP-3 and MMP-9.The 3 proteins can be used for the diagnosis of gastric cancer patients,and it also avail to make the judgment the prognosis of patients with gastric carcinoma.
