Comparative Study of Anti-oxidative Effects of Tibetan Folk Medicine Erigeron multiradiatus during Plant Growth
10.3969/j.issn.1674-6384.2011.03.007
- Author:
Zhifeng ZHANG
;
Yuan LIU
;
Pei LUO
;
Hao ZHANG
- Publication Type:Journal Article
- Keywords:
anti-oxidative activity;
developmental stage;
endothelial cells;
Erigeron multiradiatus;
hydrogen peroxide
- From:
Chinese Herbal Medicines
2011;03(3):207-213
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of a potential anti-oxidative plant,Erigeron multiradiatus(Asteraceae),plant materials from naturally distributed high-altitude populations at different stages of life cycle were collected.Methods Fifteen extracts obtained from the Ganzi region(Sichuan,China)were studied to assess their radical-scavenging ability on 1,1-diphenyl-2-picrylhydrazyl radicals and reducing power ability.Moreover,considering that anti-oxidants and free radical scavengers can also exert protective effect on endothelial cells from oxidative injury,these extracts were also evaluated for their anti-oxidative activity against cellular injury in the cultured human endothelial cell line(ECV304)induced by hydrogen peroxide(H<,2>O<,2>).Results All the extracts had radical-scavenging and/or reducing power ability,and the most active extract was found during flowering whereas the lowest appeared during vegetative growth period.The accumulation of anti-oxidative compounds was found to be affected by the altitude of growth environment.Total flavonoid content assay was also performed to support this outcome.Furthermore,these extracts also exhibited different effects on attenuating H<,2>O<,2>-induced cytotoxicity and inhibiting lipid peroxidation and LDH leakage from endothelial cells.Conclusion E.multiradiatus may be an important natural anti-oxidant and this property may contribute to verifying the utilization of this plant in Tibet folk medicine.
