Development of HPLC Method to Evaluate Drug-processing Technique of Eucommiae Cortex
10.3969/j.issn.1674-6384.2011.03.009
- Author:
Jialong WANG
;
Erwei LIU
;
Shuai WU
;
Zhaoye YIN
;
Yi ZHANG
- Publication Type:Journal Article
- Keywords:
Eucommia ulmoides;
genipin;
geniposide;
geniposidic acid;
processing technique;
RP-HPLC
- From:
Chinese Herbal Medicines
2011;03(3):221-225
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a RP-HPLC method investigate the processing technique and mechanism of Eucommiae Cortex.Methods The RP-HPLC method was applied to simultaneously determining six ingredients,geniposidic acid,geniposide,genipin,chlorogenic acid,(+)-pinoresinol-di-β-D-glucopyranoside,and(+)-syringaresinol-di-β-D-glucopyranoside,in the different processed barks of Eucommia ulmoides.Results The valid method with good accuracy could be well used to study the processing technique of E.ulmoides;Besides,target ingredients in E.ulmoide were decreased within 6 h when they were processed.Conclusion Established RP-HPLC is a reliable method which could be used to research the processing technique of the barks ofE.ulmoides.Moreover,the result of this study could be provided with significant evidence of processed barks of E.ulmoides.
