Simultaneous Determination of Seven Flavonoids in Aerial Parts of Artemisia frigida by HPLC
10.3969/j.issn.1674-6384.2012.03.013
- Author:
Qinghu WANG
;
Wuliji AO
;
Wenquan TAI
- Publication Type:Journal Article
- Keywords:
Artemisia frigida;
Compositae;
flavonoids;
HPLC;
simultaneous determination
- From:
Chinese Herbal Medicines
2012;04(3):252-258
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for the determination of seven flavonoids from the aerial part of Artemisia frigida.Methods Hypersil ODS-2 (300 mm× 4.6 mm,5 μm) column was used,with acetonitril-0.2％ phosphoric acid (gradient elution) as a mobile phase,and the detection wavelength was at 283 nm with flow rate at 1mL/min.Results All calibration curves showed good linear regression (r ＞ 0.9990) within the tested range.All average recovery was more than 98.00％ and RSD was less than 3.0％ (n =6).Conclusion The method is steady and with good repeatability,and could be used to determine the content of flavonoids in A.frigida from different areas.
