Simultaneous Determination of Seven Alkaloids in Fufang Zhenzhu Tiaozhi Capsule by HPLC Coupled with DAD
10.3969/j.issn.1674-6384.2012.03.010
- Author:
Yuanyuan CHEN
;
Jiao GUO
;
Hui FAN
;
Lihua HUANG
- Publication Type:Journal Article
- Keywords:
alkaloids;
diode array detection;
Fufang Zhenzhu Tiaozhi Capsule;
HPLC;
traditional Chinese medicines
- From:
Chinese Herbal Medicines
2012;04(3):230-236
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a reverse-phase liquid chromatography method for the determination of seven alkaloids (magnoflorine,columbamine,jatrorrhizine,epiberberine,coptisine,palmatine,and berberine) in Fufang Zhenzhu Tiaozhi Capsule.Methods Chromatography was performed on a Dionex Acclaim C18 column (250 mm × 4.6 mm,5.0 μm) at 30 ℃.The mobile phase was composed of acetonitrile-potassium dihydrogen phosphate solution (0.015 mol/L,40:60,including 1.7 g/L sodium dodecyl sulfate and phosphoric acid used to regulate pH value to 3.0),with a flow rate of 1.0 mL/min.The detection wavelength was 270 nm.Results The calibration curves of magnoflorine,columbamine,jatrorrhizine,epiberberine,coptisine,palmatine,and berberine were linear in the range of 1.07-10.65,0.78-7.55,0.75-7.50,1.60-15.95,2.69-26.85,2.31-23.10,and 6.04-60.40 mg/mL.The average recoveries of magnoflorine,columbamine,jatrorrhizine,epiberberine,coptisine,palmatine,and berberine were 101.0％,101.2％,100.1％,100.0％,100.1％,101.1％,and 99.7％,respectively.Conclusion The method could be used for the quantitative determination of the preparation.
