Smashing Tissue Extraction and HPLC Determination of Paclitaxel and 10-Deacetylbaccatin from Taxus x media
10.3969/j.issn.1674-6384.2011.03.012
- Author:
Yanze LIU
;
Wenqin GAO
;
Jiwen WANG
;
Yu ZHANG
;
Yuqing ZHAO
- Publication Type:Journal Article
- Keywords:
10-deacetylbaccatin;
HPLC;
Paclitaxel;
smashing tissue extraction;
Taxus x media
- From:
Chinese Herbal Medicines
2011;03(3):235-238
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the extraction technology of Taxus x media by using the contents of Paclitaxel and 10-deacetylbaccatin(10-DAB),two representative active diterpene alkaloids of taxane type from T.x media,as evaluation standard.Methods The smashing tissue extraction(STE)of Paclitaxel and 10-DAB from T.x media,was investigated by comparing with ultrasonic extraction(UE)which was one of the modern technologies of extraction.Results STE was more efficient than UE,and the contents of 10-DAI3 and Paclitaxel in the extracts obtained by STE were higher than those by UE.Conclusion STE is a fast,high-performance,and energy-saving technology for the extraction of diterpene alkaloids of taxane type.STE also provides a simple,component-safe,workable,and highly efficient method for the extraction of active natural product.
