RP-HPLC Determination of 1,3-Dideoxygalactonojirimycin in Bombycis Faeces
10.3969/j.issn.1674-6384.2011.02.012
- Author:
Hui QI
;
Hui ZHAO
;
Zhenying DING
;
Yuanyuan ZHU
- Publication Type:Journal Article
- Keywords:
Bombycis Faeces;
1,3-dideoxygalactonojirimycin;
extraction;
glucosidase inhibitor;
RP-HPLC
- From:
Chinese Herbal Medicines
2011;03(2):156-158
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a simple and rapid method for the determination of 1,3-dideoxygalactonojirimycin in Bombycis Faeces, a potent glucosidase inihibitor, by HPLC. Methods A RP-HPLC method with fluorescence detection has been developed. Results The HPLC method developed in this research has a good reliability including accuracy and precision. The detection limit was less than 72 ng. Conclusion This method is sufficiently sensitive for determining 1,3-dideoxygalactonojirimycin in Bombycis Faeces and other related products.
