THE EFFECT OF AMPELOPSIN ON THE APOPTOSIS INDUCTION AND CELL GROWTH INHIBITION OF HUMAN COLON CANCER SW480 CELLS

VernacularTitle:蛇葡萄素对大肠癌细胞株 SW480增殖、凋亡的影响
Author: Haizhi MA ; Zhenjun SHI ; Zhenhuai YANG
Publication Type:Journal Article
Keywords: Ampelopsin; SW480cells; Inhibitory effect; Apoptosis
From: Modern Hospital 2015;(8):12-14
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of ampelopsin on apoptosis induction and cell growth inhibition in Human Colon cancer SW480 cells in vitro.Methods Treated with ampelopsin at several concentrations, MTT and flow cytometry was used to detect the inhibition rate and apoptotic rate of SW480 cells.Western-blot was used to investigate expression of Bcl-2 family pro-tein.Results Significant difference of cell growth inhibition rate was observed among all groups after treated with ampelopsin ( p<0.05).Cell growth inhibition rate in the low -dosage group was significantly high than that in median and high -dosage group (p<0.05), while no marked difference was observed between the median and high groups (p>0.05).The similar results were observed in the experiment on apoptosis induction.Level of Bcl-xL was significantly up-regulated.Level of Bax, Bid, Caspase-3, p-Ca-pase-9 and Caspase-9 was significantly down-regulated after treatment of ampelopsin.Conclusion Ampelopsin can inhibit cell growth and induce apoptosis of SW480.Bcl-2 family protein might be involved in the progress.