A contrastive study on chemiluminescence immunoassay and ELISA for detecting HIV antibody
10.3969/j.issn.1671-332X.2016.01.011
- VernacularTitle:化学发光免疫分析法与酶联免疫法检测艾滋病抗体的对照研究
- Author:
Muhao CHENG
;
Yeyong WANG
;
Guangbin GAO
- Publication Type:Journal Article
- Keywords:
Chemiluminescence Immunoassay;
ELISA;
HIV Antibody
- From:
Modern Hospital
2016;16(1):30-32
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the effects of Chemiluminescence immunoassay ( CLIA) and ELISA for detecting the HIV antibody in order to explore the feasibility of CLIA.Methods 3,542 AIDS high-risk people were randomly selected from February 2013 to March 2015 in our city, the serum was collected and HIV antibodies in the serum were detected by CLIA and ELISA.The detection results were confirmed by the Western blot.Results By ELISA assay, 56 serum samples were positive with a positive rate of 1.58%, while CLIA 61 were positive with a pos-itive rate of 1.72%.The detection results on 11 serum samples were inconsistent among the 3,542 serum samples by ELISA and CLIA.The positive predictive value of CLIA was 96.72%, the detection sensitivity 98.33%and specific-ity 99.94%, while the positive predictive value of ELISA was 96.43%, the detection sensitivity 90.00%and speci-ficity 99.94%.The positive predictive value, sensitivity, specificity and other indicators showed no significant differ-ence between ELISA and CLIA(P>0.05).Conclusion CLIA shows high sensitivity, specificity and accuracy and therefore it can be used for detecting HIV antibody.
