Collagen Changes of the Corpus Cavernosum in Streptozotocin-Induced Diabetic Rats and the Effects of Insulin and Aminoguanidine.
- Author:
Kun Suk KIM
1
;
Jun Hyuk HONG
;
Tai Young AHN
Author Information
1. Department of Urology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea. tyahn@amc.seoul.kr
- Publication Type:Original Article
- Keywords:
Collagen;
Diabetes mellitus;
Aminoguanidine;
Corpus cavernosum
- MeSH:
Animals;
Collagen*;
Compliance;
Connective Tissue;
Diabetes Complications;
Diabetes Mellitus;
Fluorescence;
Glycosylation;
Glycosylation End Products, Advanced;
Humans;
Hydroxyproline;
Insulin*;
Male;
Rats*;
Rats, Sprague-Dawley
- From:Korean Journal of Andrology
2001;19(1):35-39
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Diabetes changes the properties of collagen in our body tissue, increasing cross-linked collagen via nonenzymatic glycosylation and accumulation of advanced glycosylation end products. Glucose-derived cross-linked collagen formation may contribute to the development of chronic diabetic complications such as decreasing the compliance of tissue. Aminoguanidine, a hydrazine compound, prevent formation of glucose-derived collagen cross-linking. We studied the change of the properties of collagen in the corpus cavernosum by diabetes and the effects of insulin and aminoguanidine. MATERIALS AND METHODS: Diabetes was induced in male Sprague-Dawley rat and maintained for 12 weeks. Rats were divided into four groups: 1) control, 2) diabetics, 3) diabetics treated with insulin, 4) diabetics treated with aminoguanidine. Concentration of collagen in cavernosal tissue was measured by hydroxyproline content and expressed as hydroxyproline (micro gram) / dried defatted tissue (mg). The characteristic autofluorescence of glycosylated connective tissues of penile tissue was used to quantitate advanced glycosylation end products. RESULTS: The content of collagen in cavernosal tissue was not significantly different among experimental groups. Cross-linked collagen expressed as fluorescence (U/micro gram hydroxyproline) was increased in diabetic group (61.6+/-5.1) compared to control group (24.1+/-3.2) and it was decreased in both insulin-treated group (36.9+/-7.4) and aminoguanidine-treated group (37.9+/-5.8) compared to diabetic group. CONCLUSIONS: Our studies revealed that the properties of collagen in corpus cavernosum was changed by diabetes and that aminoguanidine as well as insulin prevented the diabetes-induced crossed linked collagen formation.
