The effect of microRNA-27a on U251 glioma cells
10.11904/j.issn.1002-3070.2016.04.011
- VernacularTitle:MicroRNA－27a对U251胶质瘤细胞的影响
- Author:
Guojun LIU
;
Jun ZHANG
;
Yongchun FAN
;
Qingsuo WANG
;
Wei LI
- Publication Type:Journal Article
- Keywords:
MicroRNA-27 a;
Glioma;
PPARγ;
Proliferation;
Invasiveness
- From:
Practical Oncology Journal
2016;30(4):346-350
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the effect of microRNA -27a on U251 glioma cells.Methods Over-expression or inhibition of miR -27a in U251 glioma cells were done by transient transfection of miR -27a mim-ics or AMO-27a in vitro.Cell viability was detected by MTT assay .Invasion ability of U251 was detected by tr-answell invasion assay.The level of miR-27a and PPARγwere detected by real -time PCR.Results Under in-hibition of miR-27a condition,the proliferation and invasiveness of U 251 glioma cells were decreased .The level of PPARγwas significantly increased ,whereas the level of miR-27 a was decreased .Overexpression of miR-27 a increased the proliferation and invasiveness of U 251 glioma cells and decreased the level of PPARγ.Conclusion Inhibition of miR-27a is benefit for inhibiting the proliferation and invasiveness of U 251 glioma cells.
