The expression and significance of AICARFT and mTOR in pemetrexed resistant human lung adenocar-cinoma cancer cell line A549/PEM
10.11904/j.issn.1002-3070.2015.04.010
- VernacularTitle:AICARFT 及 mTOR 在人肺腺癌亲本株 A549及培美曲塞耐药株 A549／PEM 的表达及意义
- Author:
Dan ZHANG
;
Hongyang WANG
;
Yan HUANG
;
Limin WANG
- Publication Type:Journal Article
- Keywords:
AICARFT;
mTOR;
Resistant;
A549;
A549/PEM
- From:
Practical Oncology Journal
2015;(4):337-341
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of AICARFT and mTOR in lung adenocarcinoma cell line A549 and pemetrexed resistant human lung adenocarcinoma cell line A549/PEM.Methods The distri-bution of cell cycle was evaluated by flow cytometry assay.The expression of AICARFT and mTOR in A549 and A549/PEM were detected by RT-PCR and Western blot.Results Flow cytometry analysis showed that S phase in A549/PEM was higher than that in A549((36.61 ±1.36)%vs(27.16 ±1.08)%(P=0.001)).mRNA over expression of AICARFT and mTOR in A549/PEM were detected by RT-PCR(P<0.05).Protein over expres-sion of AICARFT and mTOR in A549/PEM were detected by Western blot.(P<0.05).Conclusion High level of AICARFT and mTOR may be connected with pemetrexed acquired drug resistance in lung adenocarcinoma.The cycle of pemetrexed resistant human lung adenocarcinoma cancer cell line A549/PEM is redistributed.
