The effect and mechanism of ginseng polysaccharides injective solution on invasive ability of human breast cancer MCF-7 cells
10.11904/j.issn.1002-3070.2015.04.009
- VernacularTitle:人参多糖注射液对人乳腺癌 MCF －7细胞侵袭迁移能力的影响及机制研究
- Author:
Ying LUO
;
Jing HUANG
- Publication Type:Journal Article
- Keywords:
Ginseng polysaccharides injection;
Breast cancer;
Metalloproteinases-2;
Metalloproteinases-9
- From:
Practical Oncology Journal
2015;(4):333-336
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect and mechanism of ginseng polysaccharides injective solu-tion on invasive ability of human breast cancer MCF-7 cells.Methods Human breast cancer MCF-7 cells were treated with different concentrations of ginseng polysaccharides injections(12.5μL/mL,25μL/mL,50μL/mL or 100μL/mL),and phosphate buffer saline was served as control.The invasive ability,transfer ability and protein expression of matrix metalloproteinases-2 ( MMP-2 ) and matrix metalloproteinases-9 ( MMP-9 ) were determined via transwell matrigel invasive test,scratch test and Western Blot,respectively.Results Ginseng pol-ysaccharide injection on invasive ability and transfer ability in vitro of MCF-7 cell had significant role in inhibi-ting effect,and showed dose and time dependent.After ginseng polysaccharides injection treatment,the MMP-2 and MMP-9 protein expression significantly reduced,and showed dose and time dependent manner.Conclusion Ginseng polysaccharides injection is able to inhibit the invasion and transfer in MCF -7 cells.Moreover,theregulation of MMP -2 and MMP -9 expression may be involved in this effect.
