Effects and mechanism of Biglycan and FAK signaling pathway on the invasion and metastasis of colon cancer cells
10.11904/j.issn.1002-3070.2015.05.013
- VernacularTitle:Biglycan 及 FAK 信号通路对结肠癌细胞侵袭、转移能力的影响及机制
- Author:
Xiaohu GU
;
Jingdong XIAO
;
Xiaojing XING
- Publication Type:Journal Article
- Keywords:
Biglycan;
FAK;
Colon cancer;
Invasion;
Metastasis
- From:
Practical Oncology Journal
2015;29(5):444-449
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of Biglycan on the focal adhesion kinase( FAK) activa-tion and to explore whether it regulates the invasion and metastasis of colon cancer through FAK signaling path-way.Methods The overexpressive plasmid of Biglycan was constructed and then transfected into the colon canc-er cell line HCT116.Meanwhile,FAK inhibitor was used to treat cells.Control group(HCT116),empty plasmid group(Vector),empty plasmid and inhibitor treatment group(Vector +PF -562271),Biglycan overexpression group( Biglycan) ,Biglycan overexpression and inhibitor treatment group( Biglycan+PF-562271) were set.Twen-ty four hours after exposure to inhibitor,the expression of FAK and p-FAK in each group was detected by West-ern Blot.The invasion and metastasis of colon cancer cells was detected by transwell assay.Results Overexpres-sion of Biglycan significantly enhanced the phosphorylation level of FAK and promoted the invasion and metastasis of HCT116(P<0.01).The inhibitor of FAK PF-562271 could significantly reduce the expression of p-FAK and reverse the effect of Biglycan on the invasion and metastasis of colon cancer cells.Conclusion Biglycan reg-ulates the invasion and metastasis of colon cancer cells through activation FAK signaling pathway.
