The effect of microRNA-204 on autophagy in U251
10.11904/j.issn.1002-3070.2016.01.005
- VernacularTitle:MicroRNA-204对胶质瘤细胞U251自噬的影响
- Author:
Shuang FU
;
Guojun LIU
- Publication Type:Journal Article
- Keywords:
Glioma;
MicroRNA-204;
Bcl-2;
Autophagy
- From:
Practical Oncology Journal
2016;30(1):23-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of microRNA-204(miR-204)on autophagy in U251. Methods Inhibition of miR-204 in U251 cell lines was done by transfection of miR-204 inhibitor(AMO-204) .Cell viability was detected by MTT assay.The autophagy of U251 was tested by immunofluorescence tech-nique.The protein level of Beclin 1,LC3 and Bcl-2 was detected by Western blot.Results Cell viability was markedly increased after inhibition of miR-204 in U251 cells.The number of autophagosome was decreased.The levels of Beclin 1 and LC3 were decreased,the protein level of Bcl-2 was significant increased by transfection of AMO-204 in U251 cells(P<0.05).Conclusion MiR-204 might at least in part promote glioma via inhibi-ton autophagy,indicating that miR-204 might be a potential target for the treatment of glioma.
