Autophagy facilitates breast cancer cells to resist tamoxifen by p38/c-Jun pathway
10.11904/j.issn.1002-3070.2015.01.005
- VernacularTitle:自噬通过 p38/c -Jun 信号通路促进乳腺癌细胞对他莫昔芬耐药的研究
- Author:
Zetao WANG
;
Fulong LV
;
Jinsong WANG
- Publication Type:Journal Article
- Keywords:
Autophagy;
Tamoxifen;
LC3;
p38;
c-Jun
- From:
Practical Oncology Journal
2015;(1):22-28
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of autophagy on breast cancer cells to tamoxifen resist-ance.Methods Immunofluorescence was used to count the proportion of the cells with autophagy in MCF -7 and MCF-7/TAMR,as well as ,the average of autophagosome in each cells .Western blotting was used to detect the different expressions of p38,c-jun and LC3 in MCF-7 and MCF-7/TAMR.Stepwise selection was used to detect the duration of resistance to different doses of tamoxifen when upregulating or downregulating of LC 3.West-ern blot was facilitated to investigate changes of activity of p 38/c-jun.Rusults Compared with MCF -7,the proportion of the cells with autophagosome in MCF -7/TAMR was markedly increased ,the same as the average of autophagosome in each cells .Compared with MCF-7,LC3,especially LC3IIprotein expressive levels in MCF -7/TAMR was significantly increased .The expressive level of LC3 during the induction process of TAM resistance was negative correlated with resistant cell lines .The expressions of phosphorylated p 38 and c-Jun in MCF-7/TAMR were significantly increased .Conclusion Autophagy promotes breast cancer cells to resistant to tamox-ifen,due to downregulation of the expression of autophagy related proteins which may inhibit the generation of re -sistant breast cancer cells .This may be a viable treatment strategy for refractory breast cancer .
