The inhibitive effects of miR-124 on the proliferation of breast ac ncer cell line MCF-7 induced by pa-clitaxel
10.11904/j.issn.1002-3070.2014.04.001
- VernacularTitle:miR -124参与紫杉醇诱导的乳腺癌细胞株MCF -7生长抑制
- Author:
Wenjing LI
;
Tongbao FENG
;
Chunjian QI
- Publication Type:Journal Article
- Keywords:
miR-124;
Paclitaxel;
Breast cancer
- From:
Practical Oncology Journal
2014;(4):289-293
- CountryChina
- Language:Chinese
-
Abstract:
Objective Our study aims to investigate the inhibitive effects of miR -124 on the growth of breast cancer cell MCF -7 induced by paclitaxel .Methods MTT was used to detect the growth inhibition of MCF-7 induced by paclitaxel .Flow cytometry was used to detect the effect of paclitaxel on cell cycle .Real-time quantitative PCR(qRT-PCR) was used to detect the expressive level of miR -124,while MCF -7 cells were treated with paclitaxel .MiR-124 inhibitor was transfected into MCF -7 breast cancer cells ,and growth in-hibition was detected by MTT .Results The results showed that paclitaxel could significantly inhibit the growth of breast cancer cell line MCF -7 by blocking the G2 phase.The results from qRT-PCR showed that the relative expression of miR-124 was increased when the dosage of paclitaxel was increased .When the expression of miR-124 was inhibited ,the cell growth inhibition caused by paclitaxel was also prominently decreased .Conclusion The higher expression of miR -124 in MCF-7 induced by paclitaxel was dose dependent .And miR-124 in-hibitor can significantly influence the cell growth inhibition caused by paclitaxel .These results indicat that miR -124 plays an important role in paclitaxel -induced chemotherapy drug resistance ,and provides a new direction to solve the problem .
