Correlative study on interleukin-6,interleukin -8 and interleukin-10 with radiation-induced lung damage
10.3969/j.issn.1002-3070.2014.03.003
- VernacularTitle:IL -6、IL -8和 IL -10与放射性肺损伤相关性研究
- Author:
Ying CUI
;
Da LV
;
Wenbo QIAO
- Publication Type:Journal Article
- Keywords:
Radiation-induced lung damage;
Animal model;
Interleukin;
Cytokine;
Immunohistochem-isty
- From:
Practical Oncology Journal
2014;(3):202-206
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study radiation -induced lung damage after lung ionizing radiation and the temporal and spatial release of pro -inflammatory cytokines of interleukin -6(IL-6),interleukin-8(IL-8) and interleukin-10(IL-10)in the irradiated lung tissue.Methods BALB/C male mice weighted around 25 g were randomly divided into two groups:radiation group ( R) and control group ( C) ,with 30 mice in R group and10 mice in C group.The thorax of mice was irradiated by 6 MV X-ray with 25 Gy in 5 fractions.The mice were sac-rificed at 12 weeks post irradiation.Lung tissues were collected and embedded in paraffin .After HE staining,lung histopathological changes were detected by immunohistochemistry to detect IL -6,IL-8 and IL-10 expression in lung tissue.Results Immunohistochemistry results showed that the expressions of IL -6,IL-8 and IL-10 were mainly expressed in macrophages and inflammatory cells .The results showed that the expressions of IL -6 and IL-8 in R group were significantly higher than that in C group .IL-10 expression level was lower than C group.Conclusion After 12 weeks exposing to radiation ,cytokines of IL-6,IL-8 and IL-10 in lung tissue are associated with radiation -induced lung injury .
