The research on exprse sion of ec ll cycle and apop tosis relaet d protein in keratoaac nthoma and well-dif-ferentiated squamous cell carcinoma
10.11904/j.issn.1002-3070.2014.06.002
- VernacularTitle:角化棘皮瘤与高分化鳞状细胞癌细胞周期和细胞凋亡相关蛋白表达的研究
- Author:
Bingmei LIU
;
Jing YANG
;
Hanwen ZHANG
;
Ronggui XING
;
Lili LI
;
Liping ZHAO
;
Shi QIAO
;
Haiyan YOU
- Publication Type:Journal Article
- Keywords:
Keratoacanthoma;
Squamous cell carcinoma;
p53;
bcl-2;
Ki-67
- From:
Practical Oncology Journal
2014;(6):485-491
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expressions and significances of p 53、bcl-2、Ki-67 and apopto-sis in keratoacanthoma(KA)and well differentiated squamous cell carcinoma (WDSCC).Methods The expres-sions of p53、bcl-2、Ki-67 were detected by immunohistochemical technique in 44 cases of KA and 20 cases of WDSCC.Apoptosis was identified by using terminal deoxynucleotidyl transfers ( TdT) -mediated dUTP -biotin nick and labelling(TUNEL)method.Results The positive expressive rates of p53 in proliferative、mature and re-gressive of KA were 22.23%、26.18% and 6.52%, which was lower than the positive rate of WDSCC (41.82%).Significant differences were found between every period of KA with WDSCC in p 53 expression;The intensity of expression and pattern were similar in KA of Ki -67 and p53.There were a positive correlation be-tween p53 and Ki-67 expression rate(r=0.986,P<0.001).The bcl-2 staining showed weak expression in 1 case in WDSCC and 2 cases in KA ,but only a few positive tumor cells was limited to basal cell layer in KA .The average apoptosis rate in KA was 21.72%,which was apparently higher than in WDSCC (9.925%).There were a negative correlation between the apoptosis rate and proliferation rate of Ki -67(r=-0.824,P<0.001).Con-clusion The proliferation and apoptosis in KA can coexist .However ,the apoptosis will occupy a dominance posi-tion in regressive phase ,which results in the regression of tumor .The expressions of p53、Ki-67 and apoptosis would be a certain significance in differentiated KA and WDSCC .
