Study on the role of MAT1 in estradiol regulated MMP-9,TIMP-1 expression in ER positive breast cancer
10.11904/j.issn.1002-3070.2014.06.005
- VernacularTitle:抑制 MTA1对雌二醇调控 ER 阳性乳腺癌细胞 MMP －9、TIMP －1表达的影响
- Author:
Jing WANG
;
Hang YU
;
Peng WANG
;
Changying XIE
;
Xiuqin NI
- Publication Type:Journal Article
- Keywords:
Breast cancer;
Estrogen;
Metastasis-associated protein 1;
Matrix metalloproteinase-9;
Tis-sue inhibitor of metalloproteinases -1
- From:
Practical Oncology Journal
2014;(6):503-507
- CountryChina
- Language:Chinese
-
Abstract:
Obej ctive To investigate the role of metastasis associated protein 1(MTA1)in estrogen reg-ulated expression of matrix metalloproteinase -9(MMP-9)and tissue inhibitor of metalprotease -1(TIMP-1) in estrogen receptor( ER ) positive breast cancer cells .Methods MTA1 knockdown cell model was generated based on MCF-7breast cancer cell line by transfected with MTA 1-shRNA.The mRNA and protein level of MMP-9 and TIMP-1 in wild type MCF-7(MCF-7WT)and MCF-7MTA1-shRNA before and after 17β-estradiol ( E2) treatment were examined by Real -time PCR and Western blot respectively .Results The MTA1-shRNA showed maximally 84.9%suppression of MTA1 expression in MCF-7,suggesting a satisfied MTA 1 knockdown cell model was established for subsequent experiments .After treated with E2 for 48 h,MCF-7WT showed an incre-ment of 46%(P<0.05)and 37%(P<0.05)of the mRNA and protein level of MMP -9 and a decrement of 32.3%( P<0.05)and 18.2%(P<0.05)of TIMP-1;MCF-7MTA1-shRNA showed a decrement of 32.3%(P<0.05)and 18.2%(P<0.05)of mRNA and protein expression of MMP -9 respectively but no significant differ-ence in TIMP-1 comparing with MCF-7WT before treated with Estradiol.After E2 treatment,MCF-7MTA1-shRNA didn′t show significant change of MMP -9 except decrements of 32.3%(P<0.05)and 18.2%(P<0.05)in the mRNA and protein levels of TIMP -1.Conclusion MTA1 may be involved in the pathway by which estrogen regulated the expression of MMP -9 but not TIMP-1 in ER positive breast cancer cells .
