Comparative expression of MDR-1 and MRP in peripheral blood lymphocytes in lung cancer patients with chemotherapy treatment
10.11904/j.issn.1002-3070.2015.02.007
- VernacularTitle:肺癌患者外周血中 MDR －1及 MRP 基因在化疗前后的表达及意义
- Author:
Qingnuan KONG
;
Xingning FU
;
Xiaoyan DING
;
Xia JI
;
Weiqing HUANG
- Publication Type:Journal Article
- Keywords:
Lung cancer;
Peripheral blood;
RT-PCR;
MDR-1;
MRP
- From:
Practical Oncology Journal
2015;(2):127-132
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the significance of MDR -1 and MRP mRNA expression in periph-eral blood lymphocytes in lung cancer patients with chemotherapy treatment .Methods Peripheral blood samples were taken from each lung cancer patient before chemotherapy and three weeks after the first chemotherapy cycle . The expressions of MDR-1 and MRP were tested for 39 lung cancer cases using RT -PCR technique comparing to 20 normal control cases .Results There was no significant difference for MDR -1 mRNA and MRP mRNA ex-isting in lung cancer cases and control group (P>0.05).MDR-1 and MRP mRNA expressions were increased after treatment of chemotherapy courses in almost all pathological types of lung cancer .Furthermore,the expres-sion level in small cell lung cancer after chemotherapy was significantly higher than that before .Conclusion Chemotherapy may induce the incidence rate of MDR -1mRNA and MRP mRNA expression in lung cancer .Ade-nocarcinoma and squamous cell carcinoma are mainly intrinsic MDR while small cell lung cancer is mainly ac -quired MDR to chemotherapy .Based on the positive expression rate of MDR -1 mRNA and MRP mRNA in pe-ripheral blood ,we may evaluate the results of chemotherapy in lung cancer patients easily and conveniently .
