Extraction of Magnetosome from Acidthiobacillus ferrooxidans
10.3969/j.issn.1673-6273.2005.03.002
- VernacularTitle:Acidthiobacillus ferrooxidans中磁小体的提取
- Author:
Jianping XIE
;
Xinxing LIU
;
Wenbin LIU
;
Guanzhou QIU
- Publication Type:Journal Article
- Keywords:
Biohydrometallurgy;
Magnetotactic;
Magnetosome;
Isolation of microbe;
Acidthiobacillus ferrooxidans
- From:Progress in Modern Biomedicine
2005;5(3):7-10
- CountryChina
- Language:Chinese
-
Abstract:
In physiological property and growing environment aspects, there are some similarities between Acidthiobacillus ferrooxidans(ATF) and magnetotactic bacteria. ATF shows magnetotacxis under the microscope. The magnetosome can be extracted from ATF by using the extracting method of magnetosome from magnetotactic bacteria. The membrane of ATF was crushed by ultrasonic wave and the magnetosomes in the ATF which mainly contains Fe through chemical detection were attracted by using magnetite. After purifing with sucrose density gradient centrifugation and washing by PBS, the magnetosomes can be seen clearly through a transmission electron microscope. The results indicate there are a small amount of intracellular magnetosomes which made the ATF be magnetotactic under the applied magnetic field. It is the first time to find that ATF was magnetotactic. The magnetotaxis of ATF can be utilized and isolated by the magnetotactic properties to gain the high-performance ATF strains of mineral leaching, which have high activity and different magnetism.
